The polymorphism information content pic ranged from 0. The result of genetic diversity analysis on 299 varieties of indica inbred rice. Diversity estimates are generally lacking for many wild crop relatives. The northeastern region ner of india, comprising of arunachal pradesh, assam, manipur, meghalaya, mizoram, nagaland and tripura, is a hot spot for genetic diversity and the most probable origin of rice. Control plots of monocultured crops allowed us to calculate the effect of diversity on the severity of rice blast, the major disease of rice5. Genetic diversity analysis of rice cultivars oryza sativa. Hybrid rice suitability in west africa by raafat elnamaky 1, mamadou m. However, little study on rice landrace diversity in myanmar has been done.
There is a wide genetic diversity available in rice among and between landraces. A rice diversity panel evaluated for genetic and agro. Evaluation of genetic diversity in rice subspecies using microsatellite markers. As far as we know, the collection is one of the earliest collections for rice germplasm resources and therefore we name d it as ting s collection lu et al. Agrama 1, wengui yan2, melissa jia2, robert fjellstrom2, anna m. Genetic structure associated with diversity and geographic. Genetic diversity analysis based on genomewide singlenucleotide polymorphism snp assay of a set of indian rice cultivars including modern highyielding varieties and landraces revealed two broad groups, one with aus and the other with indica cultivars. This study assesses the genetic diversity in a collection of pigmented rice accessions from the philippines.
Detailed information about the availability of material pdf. Genetic diversity of the rice blast fungus in a disease nursery in colombia. Aug 17, 2000 control plots of monocultured crops allowed us to calculate the effect of diversity on the severity of rice blast, the major disease of rice5. Putting plant genetic diversity and variability at. Molecular marker based ssr genetic diversity analysis in deep water rice germplasms of bangladesh sadia matin1, m.
The project evaluates genotypes and phenotypes in a diverse set of rice accessions and uses association mapping to link genotype and phenotype. Using rice genetic diversity to support farmers adaptation to climate change for sustainable production and improved livelihoods in india overall objective. Multiple streams of genetic diversity in japonica rice. Sixteen informative primer pairs were selected, having high. In this study, a sample of 234 accessions of rice was genotyped at.
Evaluation of genetic diversity of localcolored rice. Pandey p, anurag pj, tiwari dk, yadav sk, kumar b 2009 genetic variability, diversity and association of quantitative traits with grain yield in rice oryza sativa l. The genetic diversity maintained in a species is considered as a function of its ecological and evolutionary history choudhury et al. Assessment of genetic diversity in rice oryza sativa l. Genetic diversity of aromatic rice germplasm revealed by ssr. Wild crop relatives are an important source of genetic diversity for crop improvement. North s 20 estimation of genetic variability and correlation for grain yield components in rice oryza sativa l. The aim of this study is to examine the genetic variation of about 500 landraces from the aus and boro ecotypes of rice that fall within the aus genetic group of rice based on 384 snp markers. Dec 11, 20 level and organization of genetic diversity of rice cultivars. Some of these tools have been developed by the rice diversity project. Assessment of genetic diversity and molecular characterization among elite rice varieties of bangladesh is very important for germplasm management, varietal identification, and dna fingerprinting. Influence of ethnic traditional cultures on genetic diversity.
Increased knowledge into the genetic diversity of any germplasm collection, therefore, enhances the possibility of crop improvement and the development of superior cultivars. The knowledge of the extent and pattern of diversity in the crop species is a prerequisite for any crop improvement as it helps breeders in deciding suitable breeding strategies for their future improvement. A total of 49 probes were selected to represent the entire rflp map at intervals of 2030 cm. Molecular characterization and genetic diversity analysis. This diversity is supposed to play an important role in rice genetic improvement. Folk rice varieties, traditional knowledge and nutritional. Evaluation of genetic diversity in rice subspecies using. The distribution patterns of some interesting observations were also carried out. However, it has other uses such as animal feed, production of alcoholic beverages such as wine, rice bran oil, fuel and manufacture of insulation materials chakravarthi and naravaneni, 2006. Mar 01, 2005 within oryza sativa, there is an ancient and wellestablished divergence between the two major subspecies, indica and japonica, but finer levels of genetic structure are suggested by the breeding history. As the major element of germplasm, genetic diversity is a natural source for rice breeding to encounter existing food requirements 8. Agrobiodiversity, ensconced in the matrix of local cultural systems has enabled indigenous societies to cope with uncertainties in food availability. Abstract assessment of the genetic diversity and population structure in temperate japonica rice germplasm used in. Genetic diversity analysis of traditional rice variety.
Putting plant genetic diversity and variability at work for breeding. Among 27 them, japonica rice is likely the group derived from the first human manipulations of this 28 species. Molecular characterization and genetic diversity analysis of. Irri seeks to understand rice genetic diversity and uncover new genes and traits in rice that will help rice producers face challenges brought about by climate change, pests and diseases, and other unfavorable conditions. Department of agricultureagricultural research service dale bumpers national rice research center, stuttgart. Ssr markers have been extensively used to identify genetic variation among rice species ren et al. Genetic diversity and differentiation in indica and japonica groups of the cultivated rice oryza sativa l. Assessment of genetic diversity of drought tolerant and. To evaluate the genetic diversity and differentiation of major groups of chinese cultivars, we calculated several population genetic parameters for both global and chinese samples. Pdf genetic diversity analysis of rice faridul islam. Ever since the very beginning of agriculture more than 10 000years ago, during the process of domestication and cultivation of crop plants, a wealth of genetic diversity has been utilized and partly preserved. Aroma is one of the most significant quality traits of rice, and variety with aroma has a higher price in the market. Level and organization of genetic diversity of rice cultivars. Analysis of genetic diversity of 90 vietnamese localcolored rice accessions was evaluated by using 40 ssr markers.
Despite extensive studies on cultivated rice, the genetic structure and subdivision of this crop remain unclear at both global and local scales. A significant amount of this genetic diversity is housed in rice gene banks around the world, with a copy also conserved in the snowy depths of svalbard, norway. The result of genetic diversity analysis on 299 varieties of indica inbred rice with. Request pdf genetic diversity and disease control in rice crop heterogeneity is a possible solution to the vulnerability of monocultured crops to disease1, 2, 3.
Genetic diversity of rice grain quality intechopen. Genetic diversity and differentiation of indica and japonica. This research was carried out to study the genetic diversity among the 50 aromatic rice accessions from three regions peninsular malaysia, sabah, and. Genetic diversity and population structure of basmati rice oryza. The isolates from the liaoning province, where rice false smut has generated different take. Genetic diversity analysis of rice germplasm in tripura state.
Our study indicated a moderate level of diversity among 16 rice genotypes. Assessment of the genetic diversity of rice germplasms. Diversity and population structure of red rice germplasm in. Genetic variability study of yield and yield related traits. Genetic diversity is a natural resource for rice breeding to meet current food demands. Phyto83n12 1427 american phytopathological society. Pdf genetic diversity studies in rice oryza sativa l. Genotyping of 192 rice lines using 61 ssrs produced a total of 205 alleles with the pic value of 0. Genetic diversity of aromatic rice germplasm revealed by. The similarity coefficients of the rice landraces fluctuated from 0. In order to ascertain the diversity for its possible utilization in rice grain quality improvement in pakistan, local rice genetic resources 475 accessions collected from 3 rice cultivation zones and other parts of the country were investigated for the seed characters.
Genetic diversity in plants 88 cultivated countries. Among them, japonica rice is likely the group derived from the first human manipulations of this species. The higher the level of genetic variation is in a population. Asian cultivated rice provides a clear picture of multiple foundations of crop diversity. Thirtyfour microsatellite markers were studied across 21 types of rice to characterize and discriminate among different varieties. Preservation of the genetic diversity of a local common carp in the agricultural heritage rice fish system weizheng rena,1, liangliang hua,1, liang guoa,1, jian zhanga, lu tanga, entao zhanga, jiaen zhangb, shiming luob, jianjun tang a,2, and xin chen acollege of life sciences, zhejiang university, hangzhou 310058, china. Genetic diversity is the foundation of the genetic improvement of crops. Group b consists of 28 isolates from beijing having a diversity of 0.
Analysis of genetic diversity and population structure of. We also evaluated the likelihood of gene flow between wild and. Agriculture relies heavily on the genetic diversity of crop plants. Genetic diversity, microsatellites, oryza sativa, rice, temperate japonica germplasm. We make use of the 3,000 rice genomes 3k rg data set, first described in 2018, to explore the genetic diversity of traditional japonica rice. Within oryza sativa, there is an ancient and wellestablished divergence between the two major subspecies, indica and japonica, but finer levels of genetic structure are suggested by the breeding history. Subramanian former director of research, tnau, coimbatore abstract rice oryza sativa l.
The objective of this study was to assess the genetic diversity among 40 cultivated varieties and five wild relatives of rice, oryza sativa l. The rice diversity project, funded by nsf, is a collaborative effort, led by susan mccouch at cornell university, to explore the genetic basis of variation in rice and its wild ancestors. The objectives of present study were to examine the extent of genetic diversity in korean rice germplasm and the relationship of genetic diversity with the pedigree genotypes of korean rice cultivars. Diversity and population structure of red rice germplasm. Studies based on the small set of rice genotypes have reported a loss in genetic diversity. Mcclung 2 1university of arkansas, rice research and extension center, stuttgart, usa. Evaluation of genetic diversity in rice subspecies. The availability of an array of molecular marker systems allowed comparing the efficiency of two of these marker systems to estimate the relationships among various taxa. Sikdar1, nayem zobayer1 1department of genetic engineering and biotechmology, school of life sciences, shahjalal. Sep 26, 2015 a set of 192 diverse rice germplasm lines were genotyped using 61 genome wide ssr markers to assess the molecular genetic diversity and genetic relatedness.
We make use of the 3,000 rice genomes 3k rg data set, first described in 2018, to 29 explore the genetic diversity of traditional japonica. Many pigmented rice landraces are still grown by ethnic communities for their nutritional and cultural value. Knowledge of genetic diversity and relationships among basmati rice genotypes. The rice genetic diversity is supplemented and supported by a wide spectrum of diversity at the species and ecosystem levels onfarm. Molecular marker based genetic diversity analysis in rice. Genetic diversity analysis of rice germplasm in tripura. Genetic diversity and classification of oryza sativa with. Research open access genetic structure and diversity of. Evaluation of genetic diversity in rice subspecies using microsatellite markers junjian ni, peter m. The numbers of polymorphic alleles ranged from 3 to 12 alleles per locus and average of 7. The studies on genetic variation, diversity and population structure of rice germplasm of north east india could be an important step for.
The rice diversity project is a collaborative effort to explore the genetic basis of variation in rice and its wild ancestors. Eizenga abstract a diverse collection of 409 asian rice oryza sativa l. Northeast rice collections are known to possess various agronomically important traits like biotic and abiotic stress tolerance, unique grain and cooking quality. Genetic diversity and population structure of rice landraces. Thus, the present investigation was intended to characterize the genetic resources of local landraces of rice oryza sativa l. Very few studies, however, have investigated the genetic diversity of aus rice in bangladesh 68. Genetic diversity and disease control in rice request pdf. Pdf the objective of present study was to evaluate the genetic polymorphism and identification of diverse parents among the 76 rice accessions using. Genetic diversity and disease control in rice nature. Genetic diversity analysis reveals importance of green.
In the present study, the genetic diversity and population structure of 141 genotypes including landraces, farmers varieties, elite cultivars and advanced breeding lines of basmati rice accession collected from nw himalayas were analyzed using 40 highly polymorphic trait linked ssr markers. A set of 192 diverse rice germplasm lines were genotyped using 61 genome wide ssr markers to assess the molecular genetic diversity and genetic relatedness. The objective of the present study was to analyze how genetic diversity is distributed within and among populations of the wild rice species oryza glumaepatula in costa rica. Dna polymorphisms amplified by arbitrary primers diversity and differentiation of indica and japonica rice detected by rflp analysis. Jan 23, 2016 genetic diversity analysis based on genomewide singlenucleotide polymorphism snp assay of a set of indian rice cultivars including modern highyielding varieties and landraces revealed two broad groups, one with aus and the other with indica cultivars. Genetic diversity and population structure of rice varieties cultivated in temperate regions. Genetic diversity and population structure of rice. Useful analysis tools in the study of genetic diversity in rice. To assess the genetic diversity of the rice, 24 intragenic molecular markers representing 17 starch synthesisrelated genes were investigated in 115 taihu basin. A rice diversity panel evaluated for genetic and agromorphological diversity between subpopulations and its geographic distribution m. While more comprehensive research into genetic variability, as well.
We determined dna fingerprints and pathotypes on international differentials for 151 isolates of the rice blast fungus pyricularia grisea that were collected from 15 rice cultivars at a blast disease breeding nursery in santa rosa, colombia. Genetic diversity and classification of oryza sativa with emphasis on. Aromatic rice cultivars constitute a small but special group of rice and are considered the best in terms of quality and aroma. Pdf genetic diversity and population structure of rice. Mackill abstract molecular markers can reveal differences among accesmolecular markers are useful tools for evaluating genetic diversity sions at the dna level and thus provide a more direct, and determining cultivar. Please view the pdf by using the full text pdf link under view to the left. Jan 24, 2020 indepth studies on the genetic diversity of crops indicate that domestication is likely a drawnout process that differs from the traditional representation of a simple rapid bottleneck. Rice is the main staple crop in india with the large number of varieties released every year. Pdf genetic diversity of rice grain quality rochie.
The genetic diversity of rice genetic variation in rice can explain both the visible differences between the different varieties and their preference for certain environmental conditions for example, whether they thrive in the lowlands or at higher altitudes. Preservation of the genetic diversity of a local common. Bare coulibaly 2, maji alhassan 1,3, karim traore 1, francis nwilene 4, ibnou dieng 5, rodomiro ortiz 6, and baboucarr manneh 1. Ensure that rural communities are able to maintain and adapt traditional rice genetic diversity and to combine such actions with the introduction of new varieties landraces. Genetic erosion in traditional rice agroecosystems in. Molecular marker based ssr genetic diversity analysis in. Assessment and utilization of the genetic diversity in. There were 600 pairs of ssr primers screened against 11 rice varieties. The high genetic diversity among rice varieties in the northeast indian region is due to combined effect of ecogeographical conditions, diverse agroecosystems, and diverse human cultural preferences.
Genetic structure associated with diversity and geographic distribution in the usda rice world collection hesham a. Snpbased analysis of genetic diversity reveals important. Multiple streams of genetic diversity in japonica rice biorxiv. Exploring the genetic diversity within traditional. Genotypingbysequencing gbs is a nextgenerationsequencing based method that takes advantage of reduced representation to enable highthroughput genotyping using a large number of snp markers. Assessing the genetic diversity of rice originating from. Diversity of some indonesia local glutinous rice oryza l. Assessment of the genetic diversity and population. Rice is mainly used as a major source of human food. Genetic diversity and population structure of rice varieties. The purpose of this study was 1 to evaluate the conservation status of rice landraces in ethnic group areas in southwest of china, 2 to clarify the differences of genetic diversity of rice landraces between exsitu conservation and onfarm conservation over the past 35 years, 3 to explore the impact of traditional cultures on higher. Variability assessment for root and drought tolerance traits and. Assessing the genetic diversity for miniaturization of crop collections to form mini core collection will lead to greater utilization of germplasm to.
A similar finding was reported by behera et al 2012 in 33 medicinal rice genotypes when accessing genetic diversity among them. The result based on rapd markers for 18 local rice varieties from tana toraja and enrekang indonesia showed a high level of diversity maulana et al. Singlenucleotide polymorphisms snps have become the genetic markers of choice in various genetic, ecological, and evolutionary studies. Genetic erosion in traditional rice agroecosystems in southern philippines. Pdf analysis of genetic diversity in rice oryza sativa l. Tai,2 jason coburn, steve kresovich and susan mccouch,3 plant breeding department, cornell university, ithaca, new york 148531901 and u. In this study, a sample of 234 accessions of rice was genotyped at 169 nuclear ssrs and two chloroplast loci.